Numerical Analysis of Impedance Spectra of Yeast Suspensions

Airton Ramos, Farias D. Heric

Abstract


Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m. 

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References


K. R. Foster and H. P. Schwan "Dielectric properties of tissues,`` in Handbook of Biological Effects of Electromagnetic Fields, 2nd ed., C. Polk and E. Postow, CRC, New York, 1995, pp. 25-102.

E. T. McAdams, A. Lackermeier, J. A. McLaughlin, D. Macken and J. Jossinet, "The linear and non-linear electrical properties of the electrode-electrolyte interface,`` Biosensors and Bioelectronics, vol. 10, 1995, pp. 67-74.

D. A. Coley, An Introduction to Genetic Algorithms for Scientists and Engineers, 1st ed., World Scientific Publishing, Singapore, 1999.

K. Kinosita and T. Y. Tsong, "Voltage-induced conductance in human erythrocyte membranes,`` Biochim. Biophys. Acta, vol. 554, pp. 479–497, Jul. 1979.

D. C. Chang, "Structure and dynamics of electric field-induced membrane pores as revealed by rapid-freezing electron microscopy,`` in Guide to Electroporation and Electrofusion,D. C. Chang, B. M. Chassy, J. A. Saunders, and A. E. Sowers, Eds. San Diego, CA: Academic, 1991, pp. 9–27.

J. Teissié, M. Golzio, and M. P. Rols, "Mechanisms of cell membrane electropermeabilization: A minireview of our present (lack of ?) knowledge,`` Biochim. Biophys. Acta, vol. 1724, pp. 270–280, Aug. 2005.

D. MiklavÄiÄ and M. Puc, "Electroporation,`` in Wiley Encyclopedia of Biomedical Engineering. M. Akay, New York: Wiley, 2006, pp. 1–11.

C. Chen, S. W. Smye, M. P. Robinson and J. A. Evans, "Membrane electroporation theories: a review,`` Medical and Biological Engineering and Computing, vol. 44, 2006, pp. 4-14.

J. C. Weaver and Y. A. Chizmadzhev, "Theory of electroporation: a review,`` Bioelectrochemistry, vol. 41: pp. 135-160, 1996.

S. D. Reed and S. Li, "Electroporation advances in large animals,`` Curr. Gene Ther, vol. 9, pp. 316–326, Aug. 2009.

J. Rice, C. H. Ottensmeier, and F. K. Stevenson, "DNA vaccines: Precision tools for activating effective immunity against cancer,`` Nat. Rev. Cancer, vol. 8, pp. 108–120, Feb. 2008.

M. Pavlin, M. Kanduser, M. Rebersek, G. Pucihar, F. X. Hart, F. R. Magjarevic, and D. Miklavcic, "Effect of cell electroporation on the conductivity of a cell suspension,`` Biophys. J., vol. 88, pp. 4378–4390, Jun. 2005.

V. Ganeva and B. Galutzov, "Electropulsation as an alternative method for protein extraction from yeast,`` FEMS Microbiology Letters, vol. 174, pp. 279-284, 1999.

V. Ganeva, B. Galutzov, N. Eynard and J. Teissié, "Electroinduced extraction of β-galactosidase from Kluyveromyces lactis,`` Appl. Microbiol. Biotechnol., vol. 56, pp. 411–413, 2001.

A. Ramos and A. L. Schneider, "Electroporation of Kluyveromyces marxianus and β-D-galactosidase extraction,`` in Ganesh R. N. Applied Biological Engineering – Principles and Practice, Ed. Intech, Melbourne, 2012.

M. Pavlin, M. Kanduser, M. Rebersek, G. Pucihar, F. X. Hart, R. Magjarevic and D. Miklavcic, "Effect of cell electroporation on the conductivity of a cell suspension,`` Biophysical Journal, vol. 88, 2005, pp. 4378-4390.

D. O. H. Suzuki, A. Ramos, M. C. M. Ribeiro, L. H. Cazarolli, F. R. M. B. Silva, L. D. Leite and J. L. B. Marques "Theoretical and experimental analysis of electroporated membrane conductance in cell suspension,`` IEEE Transactions on Biomedical Engineering, vol. 58, pp. 3310-3318, 2011.

H. He, D. C. Chang, and Y. K. Lee, "Nonlinear current response of micro electroporation and resealing dynamics for human cancer cells,`` Bioelectrochemistry, vol. 72, pp. 161–168, Apr. 2008.

M. Khine, C. Ionescu-Zanetti, A. Blatz, L. Wang, and L. P. Lee, "Single cell electroporation arrays with real-time monitoring and feedback control,`` Lab Chip, vol. 7, pp. 457–462, Mar. 2007.

A. Ivorra, B. Al-Sakere, B. Rubinsky, and L. M. Mir, "In vivo electrical conductivity measurements during and after tumor electroporation: Conductivity changes reflect the treatment outcome,`` Phys. Med. Biol., vol. 54, pp. 5949–5963, Sep. 2009.

A. Ramos, A. L. S. Schneider, D. O. H. Suzuki and J. L. B. Marques, "Sinusoidal Signal Analysis of Electroporation in Biological Cells,`` IEEE Transactions on Biomedical Engineering, vol. 59, no. 10, pp. 2965-2973, Oct. 2012.




DOI: http://dx.doi.org/10.1590/S2179-10742013000200032

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